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Improved storage methods for epikeratoplasty.

G D Barrett1, A M Degebrodt, L N Walker

  • 1Lions Eye Institute, Perth, Western Australia.

Refractive & Corneal Surgery
|November 1, 1990
PubMed
Summary
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Osmotic agents like dextran and chondroitin sulfate can rehydrate swollen donor corneas to normal thickness. This method restores corneal ultrastructure and may improve epikeratoplasty outcomes.

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Corneal Transplantation

Background:

  • Donor cornea swelling during storage complicates refractive lenticule calculations for epikeratoplasty.
  • Current methods for managing corneal thickness may cause physical deformation.

Purpose of the Study:

  • To investigate the efficacy of osmotic agents in restoring donor cornea thickness to in vivo levels.
  • To evaluate if osmotic agents can replace physical methods for corneal thickness reduction.

Main Methods:

  • Porcine corneas stored in McCarey-Kaufman (MK) medium were treated with varying concentrations of dextran and chondroitin sulfate.
  • Corneal thickness was measured before and after osmotic agent treatment.
  • Light and electron microscopy were used to assess stromal ultrastructure.

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Main Results:

  • 15.00% dextran alone and 10.00% dextran with 1.35% chondroitin sulfate normalized corneal thickness.
  • Osmotic agents restored corneal stromal ultrastructure to that of fresh tissue.
  • Reduced physical deformation of donor tissue was observed.

Conclusions:

  • Osmotic agents are effective in reducing donor cornea thickness post-storage.
  • This approach may enhance the predictability of epikeratoplasty by minimizing tissue deformation.
  • Restoring normal corneal thickness and ultrastructure is achievable with osmotic agents.