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[Association of abnormal cyclooxygenase-2 gene expression with colorectal carcinoma metastasis]
Zu-guo Li1, Teng-fei Liu, Wei-bing Xie
1Key Laboratory of Functional Proteomics and Transcriptomics of Main Human Diseases of Guangdong Province and Ministry of Education, Department of Pathology, Nangfang Hospital, Southern Medical University, Guangzhou 510515, China. Lizg@fimmu.com
Objective:
To investigate cyclooxygenase-2 (COX-2) expression of in colorectal carcinoma cell lines and tissues and its clinical implications.
Methods:
SP immunohistochemistry was used to detect COX-2 protein in SW480 and SW620 cell lines and 50 primary colorectal carcinoma and 50 lymphoma metastasis carcinoma specimens. Real-time PCR was used to detect COX-2 mRNA expression in SW480 and SW620 cell lines.
Results:
SW480 and SW620 cell lines both highly expressed COX-2. The positive expression levels of COX-2 increased significantly in lymph node metastatic carcinoma in comparison with primary colorectal carcinoma (P<0.05). The expression COX-2 mRNA in SW620 cell line was higher than that of SW480 cell line, showing a mean expression increment of 2.268 folds in SW620 cell line relative to SW480.
Conclusion:
Elevated COX-2 expression may be associated with lymph node metastases of colorectal carcinoma.
