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[ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei)]
Objective:
The study the patterns of rDNA ITS sequence variation of 9 medicinal species of genus Bupleurum in China to find the DNA molecular markers for identification of these crude drugs.
Method:
The ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DNAssist Version 2.2.
Results:
Homologous alignment indicated that the consanguinity of the ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. For the same species, the consanguinity was higher than 99%.
Conclusion:
The ITS sequences may serve as reliable molecular markers for identification of Radix Bupleuri.
