Related Experiment Video
Updated: Jul 19, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
[Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with
Ying-ying Fan1, Li-xian Xu, Ai-dong Wen
1Department of Anesthesiology, Stomatology Hospital, Fourth Military Medical University, Xi'an 710033, China. fanyingying_229@hotmail.com
Objective:
To develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum.
Methods:
Human serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 microl of the supernatant for analysis by HPLC coupled with fluorescence detection. The analysis was performed with a C(18) reversed-phase column using a acetonitrile-water (90:10) phase delivered at 1.0 ml/min, with the excitation wavelength of 276 nm and emission wavelength of 310 nm.
Results:
The calibration curves were linear (r=0.997 5) within the concentration range of 0.05-10 microg/ml, the limit of propofol quantification was 50 ng/ml and the intra- and inter-day precisions were between -/+15%.
Conclusions:
The method is accurate, sensitive and simple for propofol determination in clinical anesthesia.
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Types of Detectors
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
