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Updated: Jul 19, 2026

Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation
10:04

Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation

Published on: January 12, 2016

A direct method for measuring mouse capillary cortical blood volume using multiphoton laser scanning microscopy.

Pascale Vérant1, Raphaël Serduc, Boudewijn Van Der Sanden

  • 1CNRS, UMR5588, Laboratoire de Spectrométrie Physique, Grenoble, France.

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
|October 26, 2006
PubMed
Summary

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Researchers developed a novel method to measure capillary cortical blood volume (CCBV) in mice using two-photon microscopy. This technique provides accurate, reproducible measurements of CCBV in vivo, crucial for understanding brain function.

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Microscopy

Background:

  • Understanding brain blood volume is vital for neuroscience and disease research.
  • Accurate measurement of local blood volume in brain tissue is challenging.

Purpose of the Study:

  • To develop and validate a direct in vivo method for measuring capillary cortical blood volume (CCBV).
  • To assess the reproducibility and sensitivity of the developed method.

Main Methods:

  • Utilized two-photon laser scanning microscopy to image fluorescently labeled blood in mouse cortex.
  • Calculated CCBV by integrating fluorescence intensity across depths, avoiding complex image segmentation.
  • Validated the method with theoretical justifications and numerical simulations.

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Last Updated: Jul 19, 2026

Quantification of Cerebral Vascular Architecture using Two-photon Microscopy in a Mouse Model of HIV-induced Neuroinflammation
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Published on: January 12, 2016

Intravital Microscopy of the Mouse Brain Microcirculation using a Closed Cranial Window
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Published on: November 18, 2010

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Main Results:

  • CCBV was measured in healthy mice, ranging from 2%+/-0.3% to 2.4%+/-0.4%.
  • The method demonstrated reproducibility and sensitivity to cortical vessel density and size.
  • The technique is rapid and robust against background noise.

Conclusions:

  • The developed method offers a direct, rapid, and accurate way to measure in vivo CCBV.
  • This technique facilitates the study of dynamic CCBV changes under various physiological and pathological conditions.
  • The approach is valuable for advancing research in brain function and disease.