Related Experiment Video
Updated: Jan 25, 2026

A Mouse Distraction Osteogenesis Model
Published on: November 14, 2018
[Expression of c-fos, OPG, OPGL in rabbit mandibular distraction osteogenesis zone]
Wei-li Ge1, Zhi-jian Xie, Jian-feng He
1The Affiliated Stomatological Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.
Objective:
To evaluate the possible signal transduction mechanism of the mechanical stress induced by the distraction procedure in osteocytes.
Methods:
An animal model of mandibular distraction osteogenesis in rabbits was established. The expressions of c-fos, OPG and OPGL were detected by ultrasensitive S-P immunohistochemical method.
Result:
At 4 and 8 days after distraction, distraction zone showed strong positive staining of c-fos, which were apparently higher than that in distraction zone of 2, 4 and 6 weeks after consolidation. At 4 and 8 days after distraction and 2 weeks after consolidation, the expression of OPG was strong, and then wore off gradually at 4 and 6 weeks after consolidation. Weak signals of OPGL could be detected at 6 weeks after consolidation only.
Conclusion:
c-fos, OPG and OPGL are important regulators in distraction osteogenesis. c-fos is interrelated with the mechanical stress induced by the distraction procedure closely, OPG promotes new bone formation, while OPGL plays a more active role in bone remodeling.
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