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Published on: September 22, 2020
[Cannabinoid receptor 1 expression and pathological changes in rat hippocampus after deprivation of rapid eye
Pei-fang Jiang1, Tao Zhu, Zhe-zhi Xia
1The Affiliated Children Hospital, College of Medicine, Zhejianng University, Hangzhou 310003, China. jpf6688@163.com
Objective:
To observe the expression of cannabinoid receptor 1 (CB1R) mRNA and pathological changes in rat hippocampus after deprivation of rapid eye movement (REM) sleep.
Methods:
Totally 42 Sprague-Dawley male rats were randomly divided into cage control (CC), tank control (TC) and the sleep deprivation groups (SD). The SD and TC rats were sacrificed at the end of 1 d, 3 d and 5 d sleep deprivation periods, respectively. The modified multiple platform methods were established for the REM sleep deprivation. CB1R mRNA was measured by reverse transcription-polymerase chain reaction (RT-PCR). The hippocampus sections of different stages were observed with electron microscope.
Result:
In SD 1 d group, the expression of CB1R mRNA was significantly increased compared with the CC, TC, SD 3 d and SD 5 d groups (P <0.05) while in SD 3 d group it was reduced. The expression of CB1R mRNA of SD 5 d group was significantly higher than that of the SD 3 d group (P <0.05). Neuron apoptosis was found in SD 3 d and SD 5 d groups.
Conclusion:
Sleep deprivation can cause brain injury with the changes of CB1R mRNA expression.
