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[A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein]
Hui Zhang1, Jin-Hui Sun, Hong-Lian Geng
1Clinical Laboratory, Oriental Hospital, Shanghai 201200, China.
Objective:
To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.
Methods:
Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.
Results:
In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.
Conclusion:
There is a correlation between La protein and HBV mRNA and the expression of HBV protein.
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