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Updated: Jul 19, 2026

Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Published on: November 24, 2010
Construction of saccharide-modified DNAs by DNA polymerase
Masayuki Matsui1, Yoshitaka Nishiyama, Shin-ichi Ueji
1Graduate School of Science and Technology, Kobe University, 3-11 Tsurukabuto, Kobe 657-8501, Japan.
Abstract:
Novel deoxyribonucleotide triphosphates bearing maltose or lactose groups were synthesized as substrates for DNA polymerase. The incorporation efficiencies of these modified substrates were investigated in both primer extension reactions and PCR. The stability and conformation of saccharide-modified dsDNAs were assessed by UV absorbance melting experiments and CD analysis. Enzymatic incorporation of saccharide-modified substrates can be used for the efficient production of saccharide-modified DNAs.
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