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Updated: Jul 19, 2026
Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
Published on: February 23, 2017
Preparation of graded zirconia hydroxyapatite composite bioceramic and its immunocompatibility in vitro
Renfu Quan1, Disheng Yang, Xudong Miao
1Department of Orthopaedics, Second Affiliated Hospital, Zhejiang University, 310009 Hangzhou, China.
Abstract:
To obtain immunocompatibility, graded zirconia-hydroxyapatite (ZrO(2)-HA) composite bioceramic and simplex ZrO(2)-HA composite bioceramic are prepared. Peripheral blood mononuclear cells (PBMCs) harvested from healthy individuals are cultured with the two ceramic extracts to assess their effect on the transformation of lymphocytes, apoptotic ratio, CD69 expression, and expression of cytokine of TNFalpha and IL-6, with or without phytohemoagglutinin (PHA)-stimulated cells. Ceramic extracts did not activate the resting lymphocytes, whereas the response of the PHA-stimulated cells was significantly modified. The PBMCs activated by graded ZrO(2)-HA composite bioceramic is noticeably smaller than that by simplex ZrO(2)-HA composite bioceramic; these results, however can only be seen under the amplified effect of PHA-stimulation.
