Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inflammatory and Anti-Inflammatory Response of the Ocular Surface After Excimer Laser Treatment.

Biomedicines·2026
Same author

Clinical and Wavefront Outcomes After Femtosecond Laser Versus Mechanical Microkeratome Lasik: A Prospective Paired-Eye Comparative Study.

Bioengineering (Basel, Switzerland)·2026
Same author

[Siderotic Cataract Eight Months After Intralenticular Metallic Foreign Body: A Case Report and Surgical Management].

Klinische Monatsblatter fur Augenheilkunde·2026
Same author

Functional and morphological alterations of light detection circuits in postmortem retina from donors with different stages of Alzheimer's-like pathology.

Communications biology·2026
Same author

Retinal layer reflectivity and thickness as OCT biomarkers for diagnosis and imaging-based stratification relative to the 4.5-h window in acute central retinal artery occlusion.

Frontiers in neurology·2026
Same author

[Recurrent spontaneous hyphema assosiated with focal iris vascular leakage-successful treatment with argon laser photocoagulation].

Die Ophthalmologie·2026

Related Experiment Video

Updated: Jul 19, 2026

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
07:59

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

Published on: October 28, 2022

Ultrastructural changes after artificial retinal detachment with modified retinal adhesion.

Peter Szurman1, Sigrid Roters, Salvatore Grisanti

  • 1Department of Ophthalmology 1, Eberhard-Karls University Tübingen, Schleichstrasse 12, 72076 Tübingen, Germany. peter.szurman@med.uni-tuebingen.de

Investigative Ophthalmology & Visual Science
|October 27, 2006
PubMed
Summary

Artificial retinal detachment during vitrectomy causes significant cellular damage. Using a preheated, calcium-free solution reduces retinal adhesiveness and minimizes damage, improving outcomes for submacular surgery.

More Related Videos

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
11:20

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Published on: June 14, 2021

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate
05:58

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

Published on: September 11, 2013

Related Experiment Videos

Last Updated: Jul 19, 2026

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
07:59

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

Published on: October 28, 2022

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
11:20

Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases

Published on: June 14, 2021

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate
05:58

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

Published on: September 11, 2013

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Surgical Innovation

Background:

  • Artificial retinal detachment is a key technique in submacular surgery.
  • Physiological retinal adhesiveness can impede surgical success and visual recovery.
  • Subretinal fluid injection may cause cellular damage, limiting visual rehabilitation.

Purpose of the Study:

  • To investigate ultrastructural changes from retinal detachment under vitrectomy conditions.
  • To identify factors that reduce retinal adhesiveness and minimize cellular damage during the procedure.

Main Methods:

  • Pigmented rabbits underwent vitrectomy with perfusion of various solutions (varying osmolarity, Ca(2+) concentration, temperature, ischemia).
  • Artificial bleb detachment was created via subretinal injection.
  • Eyes were processed for transmission and scanning electron microscopy.

Main Results:

  • Calcium-containing solutions led to strong retinal adhesion and significant photoreceptor and retinal pigment epithelial cell damage.
  • Calcium-free solutions reduced adhesion and damage; active calcium deprivation showed potential for damage.
  • Preheated (34°C) calcium-free solutions substantially reduced retinal adhesiveness and associated ultrastructural damage.

Conclusions:

  • Artificial retinal detachment under vitrectomy conditions causes substantial ultrastructural damage.
  • A preheated, calcium-free physiologic saline solution effectively reduces retinal adhesion.
  • This approach minimizes cellular damage, offering a safer method for submacular surgery.