Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T cell chemotaxis in a simple microfluidic device.

Francis Lin1, Eugene C Butcher

  • 1Laboratory of Immunology and Vascular Biology, Department of Pathology, School of Medicine, Stanford University, Stanford, California 94305, USA.

Lab on a Chip
|October 27, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GPR15-GPR15L axis controls colon natural TCRαβ cells residency and enteroendocrine cell homeostasis to calibrate metabolism.

Mucosal immunology·2026
Same author

GPR15-guided CD8<sup>+</sup> T regulatory cells control intestinal inflammation.

Nature·2026
Same author

The endogenous peptide GPR15L shapes the intestinal microbiota to counteract colitis.

Gut·2026
Same author

Differential migratory phenotypes of human neutrophils and breast cancer cells in a wireless unidirectional electric field platform.

Microsystems & nanoengineering·2026
Same author

COUP-TFII-mediated reprogramming of the vascular endothelium counteracts tumor immune evasion.

Nature communications·2025
Same author

Integration of hyperspectral imaging and transcriptomics from individual cells with SpectralSeq.

Genome research·2025
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
See all related articles

This study presents a simple microfluidic device for observing T cell chemotaxis. The device enables precise control over chemokine gradients, offering a new method for studying immune cell migration.

Area of Science:

  • Immunology
  • Cell Biology
  • Biomedical Engineering

Background:

  • T cell chemotaxis is crucial for adaptive immune responses and lymphocyte homing.
  • Previous methods for studying T cell migration lack precise control over experimental conditions.
  • Understanding T cell migration dynamics is essential for developing targeted immunotherapies.

Purpose of the Study:

  • To develop and validate a simple microfluidic device for studying T cell chemotaxis.
  • To demonstrate the device's capability in generating stable and controllable chemokine gradients.
  • To investigate human T cell migration in response to defined chemokine gradients.

Main Methods:

  • Fabrication of a "Y"-shaped microfluidic device using poly(dimethylsiloxane) (PDMS) via soft-lithography.

Related Experiment Videos

  • Generation of chemokine gradients (CCL19 and CXCL12) through diffusion using syringe pumps.
  • Experimental measurement and theoretical validation of gradient profiles within the microfluidic channel.

    • Main Results:

    • Experimentally observed gradient profiles closely matched theoretical predictions.
    • Stable chemokine gradients were maintained in the cell observation region.
    • Robust chemotaxis of human T cells was observed in response to single and competing chemokine gradients.

    Conclusions:

    • The developed microfluidic device offers a simple, flexible, and controllable platform for T cell chemotaxis studies.
    • This approach allows for precise spatial and temporal control of chemokine gradients, enabling highly parallel experimentation.
    • The microfluidic device provides a novel in vitro method for investigating lymphocyte migration, advancing the study of immune responses.