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Updated: Jul 19, 2026

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
The prospects of advanced molecular methods in the detection of periodontal pathogens: hybridization in situ
G Novotná1, K Hercík, J Janata
1Institut of Microbiology of the Academy of Sciences CR, Prague, Czech Republic. gnovotna@biomed.cas.cz
Abstract:
The paper summarizes the present state in the diagnostics of periodontal pathogens. Both the main advantages and drawbacks of the classic cultivation methods and those of the new DNA techniques are discussed. From the emerging methods of molecular diagnostics, the method of in situ hybridization is presented in more details. Its principle, various modifications of performance and possibilities of use are explained, including examples of its application in the detection of periodontal pathogens.
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