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Functional involvement of Noc2, a Rab27 effector, in rat parotid acinar cells

Akane Imai1, Sumio Yoshie, Tomoko Nashida

  • 1Department of Biochemistry, The Nippon Dental University, School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Niigata 951-8580, Japan. imaiak@ngt.ndu.ac.jp

Archives of Biochemistry and Biophysics
|October 28, 2006
PubMed

Insights

The Noc2/Rab27 complex is crucial for early stages of amylase release from exocrine cells. This study elucidates Noc2

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Exocytosis Research

Background:

  • Noc2's role in exocytosis is known in endocrine cells, but its function in exocrine cells remained uncharacterized.
  • Understanding Noc2's involvement in exocrine secretion is vital for comprehending cellular processes.

Purpose of the Study:

  • To investigate the protein expression, subcellular localization, and function of Noc2 in exocrine acinar cells.
  • To determine if Noc2, a Rab27 effector, participates in isoproterenol (IPR)-stimulated amylase release.

Main Methods:

  • Western blotting and subcellular fractionation to analyze Rab27 and Noc2 localization in apical plasma membrane (APM) and secretory granule membrane (SGM).
  • Stimulation of parotid acinar cells with isoproterenol (IPR) to observe protein translocation.
  • Functional assay using an anti-Noc2-Rab-binding-domain antibody to inhibit amylase release in permeabilized cells.

Main Results:

  • Rab27 translocated to the APM upon IPR stimulation, with levels decreasing after 30 minutes.
  • Noc2, while expressed in SGM bound to Rab27, did not translocate to the APM and its complex with Rab27 disrupted early in IPR stimulation.
  • Inhibition of the Noc2-Rab27 interaction significantly reduced IPR-stimulated amylase release.

Conclusions:

  • The Noc2/Rab27 complex plays a significant role in the initial phase of IPR-stimulated amylase release in exocrine acinar cells.
  • Noc2's function in exocrine exocytosis is linked to its interaction with Rab27 during the early stages of secretion.

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