Related Experiment Video
Updated: Jul 19, 2026
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
Heteroparental blastocyst production from microsurgically corrected tripronucleated human embryos
María-José Escribá1, Julio Martín, Carmen Rubio
1IVI Foundation, IVI University, Valencia, Spain. mjescriba@ivi.es <mjescriba@ivi.es>
Objective:
To prove the efficiency of identification and removal of one of the surplus paternal pronuclei in dispermic IVF zygotes to obtain heteroparental blastocysts.
Design:
Experimental.
Setting:
One hundred fourteen tripronucleated (3PN) embryos from conventional IVF.
Patient(S):
After informed and signed consent, the patients from Instituto Valenciano Infertilidad (IVI), Valencia, donated their abnormally fertilized embryos.
Intervention(S):
Seventy-two embryos were diploidized by microsurgical removal of the pronucleus located at the farthest position to the second polar body. Forty-two 3PN embryos served as controls.
Main Outcome Measure(S):
Survival and correction rate; in vitro development up to the blastocyst stage; X, Y, and 18 chromosome determination by triple fluorescent in situ hybridization and, inheritance analysis for 10 polymorphic repeat regions using polymerase chain reaction (PCR) amplification and sequencing.
Result(S):
Seventy-eight percent of 3PN zygotes (56/72) survived manipulation and eventually 51 zygotes had two pronuclei (71%). Forty-one percent of manipulated embryos progressed in vitro to the blastocyst stage (21/51). Fluorescent in situ hybridization analysis performed on eight manipulated embryos confirmed their diploid state; all four controls were triploid. Heteroparental inheritances were also confirmed in four of six manipulated embryos.
Conclusion(S):
Heteroparental blastocysts can be derived from corrected dispermic zygotes.
