Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A general method for bead-enhanced quantitation by flow cytometry.

Martin Montes1, Elin A Jaensson, Aaron F Orozco

  • 1Department of Medicine, Baylor College of Medicine, Houston, Texas 77030, USA.

Journal of Immunological Methods
|October 28, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The viral and host genomic landscape of human T-cell leukemia virus type I in Peru.

Virology journal·2026
Same author

Empiric azithromycin alters the upper respiratory microbiome and resistome without anti-inflammatory benefit in COVID-19.

Nature microbiology·2026
Same author

Epidemiology of adult T-cell leukemia/lymphoma (ATL) in people living with HTLV-1: A 30-year study in Peru.

PLoS neglected tropical diseases·2026
Same author

Utilization of Intensive Care Interventions in Critically Ill Patients With Candidemia Versus Bacteremia: A Multicenter Retrospective Cohort Study.

Critical care medicine·2026
Same author

Machine learning models predict long COVID outcomes based on baseline clinical and immunologic factors.

Communications medicine·2026
Same author

Evaluating COVID-19 severity prediction and immune dynamics with NULISAseq: Insights from the IMPACC study.

Journal of immunology (Baltimore, Md. : 1950)·2025
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

Single bead-enhanced cytofluorimetry (SBEC) enables accurate absolute cell quantitation using flow cytometry. This simple, inexpensive method quantifies CD4+ T cells, eosinophils, and apoptotic cell fragments in complex samples without special software.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Flow cytometry offers relative cell quantitation but lacks absolute accuracy due to technical limitations.
  • Existing absolute quantitation standards for CD4+ T cells are costly, complex, and have limited applications.
  • Validation in complex biological samples, especially for non-T cells, remains a challenge for current methods.

Purpose of the Study:

  • To develop a simple, cost-effective method for absolute cell quantitation using flow cytometry.
  • To validate the use of polystyrene beads for enumerating various cell types and particles.
  • To establish a broadly applicable technique for routine absolute cell counting.

Main Methods:

  • Addition of known quantities of polystyrene fluorescence standardization beads to cell samples.

Related Experiment Videos

  • Implementation of single bead-enhanced cytofluorimetry (SBEC) for absolute quantitation.
  • Enumeration of CD4+ T cells, eosinophils, and apoptotic cell fragments.

    • Main Results:

    • SBEC accurately quantifies CD4+ T cells in complex cell samples.
    • The method effectively enumerates diverse entities, including eosinophils and apoptotic cell fragments.
    • SBEC demonstrated comparable accuracy to proprietary methods but with greater simplicity and lower cost.

    Conclusions:

    • Single bead-enhanced cytofluorimetry (SBEC) provides an accurate and accessible method for absolute cell quantitation.
    • The technique is versatile, suitable for various cell types and particles with different characteristics.
    • SBEC is a cost-effective and user-friendly alternative for routine flow cytometry applications.