Heterocycles related to nucleotides. XI. An organomercurial column chromatography for thiouridine

By the organomercurial column: chromagel or Sephadex coupled to p-aminophenylmercury chloride, 4- and 2-thiouridine were selectively separated from the mixtures with major nucleotides. Oligonucleotides containing 4-thiouridilic acid were also separated from the ribonuclease-T1 digests of unfractionated transfer ribonucleic acid of E. coli.