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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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A simple fluorescent biosensor for theophylline based on its RNA aptamer.

C J Rankin1, E N Fuller, K H Hamor

  • 1Department of Chemistry and Biochemistry, University of North Carolina-Greensboro, Greensboro, North Carolina 92110, USA.

Nucleosides, Nucleotides & Nucleic Acids
|October 28, 2006
PubMed
Summary

A new fluorescent biosensor detects theophylline, a bronchodilator, using RNA aptamers. This sensitive sensor offers improved detection for clinical applications.

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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
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Last Updated: Jul 19, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

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Published on: October 6, 2022

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
08:11

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Published on: August 9, 2022

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Theophylline is a critical bronchodilator medication.
  • Its narrow therapeutic index necessitates precise monitoring.
  • Existing detection methods can be complex or lack sensitivity.

Purpose of the Study:

  • To develop a simple and sensitive fluorescent biosensor for detecting theophylline.
  • To utilize the specificity of RNA aptamers for theophylline detection.
  • To enhance the dynamic range and clinical relevance of the biosensor.

Main Methods:

  • A theophylline-binding RNA aptamer was hybridized with a fluorescently labeled DNA strand (FL-DNA).
  • The resulting RNA:DNA hybrid's fluorescence was measured to quantify theophylline.
  • A dual-labeled DNA strand (FL-DNA-Q) with a quenching dye was synthesized to improve performance.

Main Results:

  • The biosensor demonstrated high selectivity for theophylline over caffeine.
  • The sensor was sensitive to theophylline concentrations as low as 0-2 muM.
  • Incorporating a quenching dye increased the biosensor's dynamic range from 1.5-fold to 4-fold.

Conclusions:

  • A simple, selective, and sensitive fluorescent biosensor for theophylline detection has been successfully developed.
  • The RNA aptamer-based biosensor shows promise for clinical monitoring of theophylline.
  • Further optimization could enhance its utility for therapeutic drug monitoring.