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Related Experiment Videos

qPrimerDepot: a primer database for quantitative real time PCR.

Wenwu Cui1, Dennis D Taub, Kevin Gardner

  • 1Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, Bethesda, MD 20892-4605.

Nucleic Acids Research
|October 28, 2006
PubMed
Summary

qPrimerDepot offers optimized primers for human and mouse genes, simplifying gene expression studies. This database ensures uniform amplification conditions, reducing background noise for reliable real-time PCR results.

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Area of Science:

  • Molecular Biology
  • Genomics

Background:

  • High-throughput gene expression studies using real-time PCR require consistent amplification conditions for multiple targets.
  • Optimizing primers for diverse genes under uniform conditions is challenging.

Purpose of the Study:

  • To develop a comprehensive primer database for human and mouse RefSeq genes.
  • To provide primers designed for unified annealing temperatures and minimized genomic DNA contamination.

Main Methods:

  • Development of the qPrimerDepot database.
  • Primer design strategy focusing on flanking large introns for intron-bearing genes.
  • Ensuring primers amplify targets under a unified annealing temperature.

Main Results:

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  • qPrimerDepot provides optimized primers for all human and mouse RefSeq genes.
  • Primers are designed for uniform amplification conditions.
  • Intron-flanking primer design minimizes genomic DNA contamination.

    • Conclusions:

    • qPrimerDepot facilitates high-throughput gene expression analysis by standardizing primer performance.
    • The database simplifies experimental setup for real-time PCR studies.
    • Optimized primers reduce background noise and improve data reliability.