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Updated: Jul 19, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy.

Frédéric Montfort¹, Tristan Colomb, Florian Charrière

¹Institut d'Optique Appliquée, Ecole Polytechnique Fédérale de Lausanne, Switzerland. frederic.montfort@a3.epfl.ch

|October 28, 2006

Summary

This study introduces multi-wavelength digital holographic microscopy for submicrometer 3D imaging. The technique achieves high-resolution tomographic reconstruction by summing phase-adjusted wavefronts, enabling detailed slice imaging of microscopic specimens.

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

Optical Microscopy
Holography
Tomographic Imaging

Background:

Digital holographic microscopy (DHM) enables label-free imaging of microscopic samples.
Traditional DHM often lacks precise depth-sectioning capabilities for complex 3D structures.
Submicrometer resolution imaging is crucial for detailed analysis of micro-objects.

Purpose of the Study:

To develop a novel tomographic imaging method using multiple wavelengths in DHM.
To achieve submicrometer resolution and precise depth sectioning of microscopic specimens.
To enable numerical scanning of objects in depth for detailed 3D reconstruction.

Main Methods:

Recording off-axis digital holograms at multiple, k-domain equally separated wavelengths (480-700 nm).
Numerical reconstruction of complex object wavefronts using convolution formulation.
Phase adjustment and summation of reconstructed wavefronts for constructive interference in a target plane.

Main Results:

Demonstrated tomographic reconstruction by constructive and destructive interference of summed wavefronts.
Achieved object sectioning into slices with a thickness of 725 nm.
Validated the method through simulations and experimental results using 20 wavelengths.

Conclusions:

The proposed multi-wavelength DHM technique provides effective submicrometer tomographic imaging.
This method allows for precise depth scanning and high-resolution 3D reconstruction of microscopic samples.
The technique offers a powerful tool for advanced materials science and biological imaging applications.