Related Experiment Video
Updated: Jan 20, 2026

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
Published on: July 16, 2019
Induction of nitrite production in mouse spleen cells by immunization
K Takagi1, H Nakagami, T Nakano
1Department of Environment Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Japan.
Abstract:
Changes of nitrite production in mouse spleen cells of in vitro secondary antibody response were investigated. Mouse spleen cells immunized with gamma globulin fraction of rat serum produced nitrite 3 days after in vitro challenging with the same antigen. Nitrite production of rabbit IgG-challenged spleen cells was found to be about 2.9-times higher than that of spleen cells primed with the gamma globulin fraction of rat serum. Nitrite production in this system was completely suppressed by T cell depletion (99.7% inhibition). Furthermore, nitrite production in these cells significantly decreased by addition of anti-interferon gamma antibody (62.9% inhibition). These data indicate that nitrite production in antigen-immunized spleen cells is affected with the immunogenicity of an antigen and regulated by T cells, especially interferon (IFN) gamma.
More Related Videos
Related Concept Videos
09:11Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
08:04A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
08:47Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
05:18Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
04:47A Mouse Model of Vascularized Heterotopic Spleen Transplantation
03:06Developing a Humanized Mouse Model with Human Liver Cells and Immune Cells

