Related Experiment Video
Updated: Jul 19, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
Published on: November 26, 2013
Engineering of GAL1 promoter-driven expression system with artificial transcription factors
1College of Medicine, Pochon CHA University, 606-16 Yeoksam1-dong, Gangnam-gu, Seoul 135-081, South Korea. pks@chahealthsystems.co.kr
Abstract:
We isolated and characterized artificial transcription factors (ATFs) that functionally activate GAL1 promoter in yeast. These ATFs transformed the yeast galactose-dependent GAL1 promoter system into a galactose-independent one. The ATFs were identified by screening a combinatorial library of zinc finger-containing transcription factors for components that activated the transcription of a reporter gene under the control of a truncated GAL1 promoter from which the GAL4p-binding sites were deleted. We also showed that these ATFs activate transcription from GAL1 promoter by binding directly to specific sequence elements in the promoter.
More Related Videos
Related Concept Videos
Reporter Genes
Commonly used reporter...
Transcription Factors
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
General Transcription Factors
RNA Polymerase II Accessory Proteins

