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Quantification of γH2AX Foci in Response to Ionising Radiation
Published on: April 6, 2010
Microarray analysis of radiation response genes in primary human fibroblasts
Enikö Kis1, Tünde Szatmári, Márton Keszei
1Department of Molecular and Tumor Radiobiology, NCPH-Frederic Joliot-Curie National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary.
International Journal of Radiation Oncology, Biology, Physics
|October 31, 2006
Summary
Ionizing radiation triggers early gene expression changes in human cells, impacting DNA repair and cell cycle pathways. These findings aid in understanding radiation response and identifying susceptible individuals.
Area of Science:
- Molecular Biology
- Genomics
- Cell Biology
Background:
- Primary human fibroblasts are crucial models for studying cellular responses to radiation.
- Understanding early transcriptional events post-irradiation is key to elucidating damage repair mechanisms.
Purpose of the Study:
- To identify early transcriptional responses in human fibroblasts following gamma-radiation exposure.
- To elucidate cellular pathways involved in DNA damage correction and cellular response.
Main Methods:
- Irradiation of primary human fibroblasts with 2 Gy gamma-radiation.
- RNA isolation 2 hours post-irradiation for transcriptional analysis using genome-wide microarrays.
- Quantitative real-time polymerase chain reaction (RT-PCR) to study time- and dose-dependent responses.
Main Results:
- Approximately 200 genes exhibited transcriptional alterations in response to ionizing radiation.
- Thirty consensus genes showed consistent responses across all investigated cells, with 28 up-regulated and 2 down-regulated.
- Consensus genes were functionally categorized into DNA damage response, cell cycle regulation, apoptosis, and signaling pathways, with many linked to p53.
Conclusions:
- The identified transcriptional alterations provide insights into early radiation responses in human fibroblasts.
- The findings may contribute to developing biomarkers for identifying individuals susceptible to radiation exposure.
