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Updated: Jul 19, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
Published on: December 19, 2018
Functional interactions between calmodulin and estrogen receptor-alpha
1Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, United States.
Abstract:
The effects of estrogen in breast epithelial cells are mediated primarily via estrogen receptor alpha and estrogen receptor beta (ERalpha and ERbeta). Accumulating evidence implicates a role for Ca2+ and calmodulin in breast carcinoma, as well as in the function of ERalpha. Calmodulin contributes to the regulation of both ERalpha degradation and ERalpha-mediated transcriptional activation. Recent data will be summarized in this review. Models to explain the molecular mechanisms by which calmodulin modulates ERalpha function are proposed.
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