Related Experiment Video
Updated: Jul 19, 2026
14:48
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
Published on: May 8, 2021
Automated post-collection concentration for purified preparative fractions via solid phase extraction
Joan Stevens1, Mark Crawford, Greg Robinson
1Gilson, Inc., 3000 Parmenter Street Middleton, WI 53562, USA. jstevens@gilson.com <jstevens@gilson.com>
Journal of Chromatography. A
|October 31, 2006
Summary
Solid phase extraction (SPE) offers an alternative to fraction evaporation in preparative HPLC. This method efficiently concentrates purified compounds, achieving high recovery rates for natural antioxidants.
Area of Science:
- Analytical Chemistry
- Natural Product Chemistry
Background:
- Preparative High-Performance Liquid Chromatography (HPLC) is crucial for compound purification.
- Fraction evaporation is a time-consuming bottleneck in preparative HPLC workflows.
Purpose of the Study:
- To evaluate Solid Phase Extraction (SPE) as an alternative to fraction evaporation for concentrating HPLC fractions.
- To assess the efficiency and recovery rates of SPE for specific natural compounds.
Main Methods:
- Fractions collected from preparative HPLC were diluted and passed through SPE cartridges.
- SPE cartridges were dried with nitrogen and eluted with 100% organic solvent.
- Recovery rates were determined for rosmarinic acid and carvacrol.
Main Results:
- SPE effectively removes water and reduces the volume of HPLC fractions.
- High recovery rates (95-98%) were achieved for rosmarinic acid and carvacrol.
- The SPE method did not present an appreciable breakthrough for the tested compounds.
Conclusions:
- Solid Phase Extraction (SPE) is a viable and efficient alternative to fraction evaporation for concentrating purified compounds from preparative HPLC.
- This method streamlines purification processes and maintains high compound recovery.
