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Updated: Jul 19, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
Published on: May 17, 2020
Transgenic animal production and animal biotechnology
J M Robl1, Z Wang, P Kasinathan
1Hematech Inc, Sioux Falls, SD 57106, USA. jrobl@hematech.com
Abstract:
Considerable progress has been made in methods for production of transgenic livestock; beginning with pronuclear microinjection over 20 years ago. New methods, including the use of viral vectors, sperm-mediated gene transfer and somatic cell cloning, have overcome many of the limitations of pronuclear microinjection. It is now possible to not only readily make simple insertional genetic modifications, but also to accomplish, more complex, homozygous gene targeting and artificial chromosome transfer in livestock.
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