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Updated: Jul 19, 2026
Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against Bacillus anthracis and Other Encapsulated Pathogens
Published on: May 19, 2020
Qualification and performance characteristics of a quantitative enzyme-linked immunosorbent assay for human lgG
Cheryl L Selinsky1, Vanessa D Whitlow, Larry R Smith
1The Biodesign Institute at Arizona State University, Tempe, AZ, USA.
Abstract:
The contribution of Bacillus anthracis lethal factor (LF)-specific immune responses to protection against anthrax disease in humans remains incompletely defined due, in part, to a paucity of qualified reagents and a lack of standardized serological assays. Toward this end, we have identified and characterized suitable positive quality control and standard reference sera and developed, optimized, and qualified an enzyme-linked immunosorbent assay (ELISA) to measure LF-binding IgG. Herein we describe the performance characteristics of this ELISA and propose criteria for its use in the detection and quantification of anti-LF IgG in human serum.
