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Related Experiment Videos

A solid-phase microextraction chamber method for analysis of manure volatiles.

Daniel N Miller1, Bryan L Woodbury

  • 1USDA-ARS, U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, NE 68933, USA. dmiller15@unl.edu

Journal of Environmental Quality
|October 31, 2006
PubMed
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A new method using dynamic flux chambers and GC-MS simplifies analyzing livestock odor compounds. This technique effectively compares volatile emissions from cattle and swine feces, aiding in odor management strategies.

Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Agricultural Science

Background:

  • Livestock operations generate complex odors from volatile compounds.
  • Current methods for analyzing these volatiles are time-consuming and require specialized equipment.
  • Accurate identification and quantification of odorants are crucial for environmental management.

Purpose of the Study:

  • To develop and validate a novel, efficient method for analyzing volatile organic compounds (VOCs) emitted from livestock feces.
  • To compare the odorous compound profiles of cattle and swine feces using the new method.
  • To establish a standardized protocol for reproducible odorant emission analysis.

Main Methods:

  • Utilized a dynamic flux chamber coupled with solid-phase microextraction (SPME) and gas chromatography-mass spectroscopy (GC-MS).

Related Experiment Videos

  • Optimized method parameters including sample temperature, surface area, airflow, SPME exposure time, and cleaning procedures.
  • Compared relative emissions from fresh and incubated cattle and swine feces under varied storage conditions.

    • Main Results:

    • Successfully detected a wide range of VOCs, including alcohols, volatile fatty acids, and sulfides.
    • Established a reproducible protocol for comparing relative emissions from different fecal samples.
    • Identified significant differences in volatile profiles based on animal species, diet, incubation, and storage temperature.

    Conclusions:

    • The developed dynamic flux chamber-SPME-GC-MS method provides an efficient and effective approach for analyzing livestock odorants.
    • This method allows for meaningful comparisons of volatile emissions, aiding in understanding and mitigating livestock-related odors.
    • The findings highlight the influence of various factors on fecal volatile compound profiles, important for manure management.