Analysis and screening of yeast artificial chromosome libraries by filter hybridization

Large-scale genome mapping and sequencing projects nowadays rely on robotic systems for yeast artificial chromosome (YAC) library processing, handling, and analysis. The advantages are obvious. Robots are fast, less labor intense, and the data reproducibility is high. However, there may be cases in which automated systems are not available or are unnecessary, for example, if the YAC library is small. In this chapter, we describe a simple method to process YAC libraries manually and screen them by conventional filter hybridization.