Related Experiment Video
Updated: Jul 19, 2026
10:51
Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
Cross-talk between nuclear receptors and nuclear factor kappaB
K De Bosscher1, W Vanden Berghe, G Haegeman
1Laboratory for Eukaryotic Gene Expression and Signal Transduction (LEGEST), Department of Molecular Biology, Ghent University, Gent, Belgium. karolien.debosscher@ugent.be
Oncogene
|October 31, 2006
Summary
Nuclear receptors (NRs) can inhibit nuclear factor kappaB (NF-kappaB), a key factor in inflammation and cell growth. Understanding this cross-talk offers new therapeutic strategies for inflammatory and tumor conditions.
Area of Science:
- Molecular biology
- Cellular signaling
- Pharmacology
Background:
- Nuclear receptors (NRs) regulate gene expression.
- Nuclear factor kappaB (NF-kappaB) is a critical transcription factor in inflammation, proliferation, and apoptosis.
- Cross-talk between NRs and NF-kappaB influences cellular processes.
Purpose of the Study:
- To review the molecular mechanisms of cross-talk between NRs and NF-kappaB.
- To discuss the biological relevance of this interaction.
- To explore selective NR modulation for therapeutic applications.
Main Methods:
- Literature review of studies on NR-NF-kappaB interactions.
- Analysis of molecular mechanisms governing cross-talk.
- Discussion of selective NR modulation strategies.
Main Results:
- Specific NRs, including glucocorticoid receptor, estrogen receptor, and PPAR, inhibit NF-kappaB activity.
- This inhibition impacts genes involved in inflammation, cell proliferation, and apoptosis.
- Selective NR modulation presents a promising approach for targeted therapies.
Conclusions:
- The cross-talk between NRs and NF-kappaB is a significant regulatory pathway.
- Targeting this interaction offers potential for developing novel anti-inflammatory, anti-tumor, and immune-modulatory drugs.
- Future research in selective NR modulation could revolutionize NR-based therapies.
Related Concept Videos
01:31Transducer Mechanism: Nuclear Receptors
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
01:45Regulation of Nuclear Protein Sorting
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
01:26Signal Transduction: Overview
Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
Typically, signal transduction involves three...
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...