Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A model for decoding resistance in precision oncology: acquired resistance to FGFR inhibitors in cholangiocarcinoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2024
Same author

Staging Chronic Hepatitis B Related Liver Fibrosis with Diffusion-weighted MRI-based Virtual Elastography: Comparisons with Serum Fibrosis Indexes.

Clinical radiology·2024
Same author

Terahertz Imaging Detects Oral Cariogenic Microbial Domains Characteristics.

Journal of dental research·2024
Same author

Trimethylamine Induced Chronic Kidney Injury by Activating the ZBP1-NLRP3 Inflammasome Pathway.

Physiological research·2024
Same author

[LncRNA MAGI2-AS3 enhances cisplatin sensitivity of non-small cell lung cancer cells by regulating the miR-1269a/PTEN/AKT pathway].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2024
Same author

[Research prospect of cyclic nucleotide phosphodiesterase 3/4 inhibitors in chronic obstructive pulmonary disease].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2024

Related Experiment Video

Updated: Jul 19, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

miR-21-mediated tumor growth.

M-L Si1, S Zhu, H Wu

  • 1Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, Springfield, IL 62794, USA.

Oncogene
|October 31, 2006
PubMed
Summary

MicroRNAs (miRNAs) are small RNAs regulating genes. This study shows that inhibiting miR-21, a microRNA overexpressed in breast tumors, suppressed tumor growth and increased apoptosis, suggesting miR-21 is an oncogene and potential therapeutic target.

More Related Videos

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

Related Experiment Videos

Last Updated: Jul 19, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
09:48

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases

Published on: August 23, 2024

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are non-coding RNA molecules regulating gene expression post-transcriptionally.
  • Aberrant miRNA expression is implicated in human cancers, but their specific role in tumorigenesis requires further investigation.
  • The function of specific microRNAs, such as miR-21, in tumor growth is not fully understood.

Purpose of the Study:

  • To investigate the role of miR-21 in breast cancer progression.
  • To determine if inhibiting miR-21 affects breast cancer cell growth and tumor development.
  • To identify potential therapeutic strategies targeting miR-21 in breast cancer.

Main Methods:

  • miRNA expression profiling in matched normal and breast tumor tissues using TaqMan real-time PCR miRNA array.
  • Transfection of breast cancer MCF-7 cells with anti-miR-21 oligonucleotides.
  • Evaluation of cell growth, apoptosis, and proliferation in vitro and in a xenograft mouse model.

Main Results:

  • miR-21 was significantly overexpressed in breast tumors compared to normal tissues.
  • Anti-miR-21 treatment suppressed breast cancer cell growth in vitro and tumor growth in vivo.
  • Inhibition of miR-21 led to increased apoptosis and decreased cell proliferation.
  • Downregulation of the anti-apoptotic gene Bcl-2 was observed in anti-miR-21-treated cells.

Conclusions:

  • miR-21 acts as an oncogene in breast cancer.
  • miR-21 modulates tumorigenesis, potentially through regulating Bcl-2.
  • miR-21 represents a promising novel therapeutic target for breast cancer treatment.