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Updated: Jul 19, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
Electrochemical detection of glutathione using redox indicators
Eden Joy Pacsial-Ong1, Robin L McCarley, Weihua Wang
1Department of Chemistry and Center for Biomodular Multiscale Systems, Louisiana State University, 232 Choppin Hall, Baton Rouge, Louisiana 70803-1804, USA.
Abstract:
The nucleophilic addition of the aminothiols homocysteine (HCY), cysteine (CYS), and glutathione (GSH) to the electrogenerated quinone of fluorone black (1) via the ECE mechanism is reported. It is demonstrated that 1selectively reacts with GSH to form the bis-GSH adduct, 1-(GSH)(2), while only the monothiol adducts were generated in the presence of HCY and CYS (1-HCY and 1-CYS, respectively). The more anodic E(pa) of 1-(GSH)2 relative to 1 and 1-GSH (DeltaE(pa) approximately +0.14) is the voltammetric signature that allows the discrimination of GSH from HCY and CYS. It is also shown that the presence of structurally similar aminothiols-HCY and CYS-posed no interference to the signature voltammetric response of 1-(GSH)2.
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