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Related Experiment Video

Updated: Jul 19, 2026

Kinematic Analysis of Cell Division and Expansion: Quantifying the Cellular Basis of Growth and Sampling Developmental Zones in Zea mays Leaves

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Published on: December 2, 2016

Salinity-induced decrease in NADPH oxidase activity in the maize leaf blade elongation zone.

Andrés A Rodríguez¹, H Ramiro Lascano, Dolores Bustos

¹IFFIVE-INTA, Camino a 60 Cuadras Km 5 1/2, 5119 Córdoba, Argentina.

Journal of Plant Physiology

|November 1, 2006

Summary

Salinity reduces maize leaf growth by decreasing reactive oxygen species (ROS) production. This study identifies plasma membrane NADPH oxidase as the ROS-generating activity inhibited by salt stress.

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Area of Science:

Plant Physiology
Molecular Biology
Biochemistry

Background:

Salinity stress constrains maize leaf expansion.
This constraint is linked to reduced reactive oxygen species (ROS) production.
Previous work showed ROS addition alleviates this effect, and the impact of NaCl is salt-specific, not osmotic.

Purpose of the Study:

To investigate the underlying causes of reduced ROS production under salinity in maize leaves.
To identify the specific ROS-generating enzyme affected by NaCl.

Main Methods:

Experiments were conducted using protoplasts and membrane fractions to assess superoxide anion (O(2)(-)) production.
Assays included the use of NaCl, diphenyleneiodonium (DPI), EGTA, and verapamil (a Ca(2+) channel inhibitor).
Native PAGE (ND-PAGE) and immunoblotting with an antibody against a gp91(phox) homolog were used to identify the enzyme responsible for O(2)(-) generation.

Main Results:

NaCl significantly inhibited O(2)(-) production in cell-free systems.
Reduced Ca(2+) supply, via EGTA and verapamil, also inhibited O(2)(-) generation.
The enzyme responsible for O(2)(-) production, identified via ND-PAGE and antibody recognition, is consistent with plasma membrane NADPH oxidase.

Conclusions:

The reduction in ROS levels under salinity is not due to increased antioxidant activity in the apoplast.
NaCl directly inhibits the activity of plasma membrane NADPH oxidase.
Plasma membrane NADPH oxidase is a key component in the salinity-induced reduction of ROS production affecting maize leaf growth.