Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 19, 2026

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Published on: March 4, 2016

Diagnostic yield associated with multiple simultaneous skeletal muscle biopsies.

Richard A Prayson¹

¹Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.

American Journal of Clinical Pathology

|November 1, 2006

Summary

Biopsying two muscles simultaneously aids diagnosing skeletal muscle disorders, especially inflammatory myopathies, by increasing diagnostic yield. This approach is crucial as single biopsies may miss treatable conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Preventing Microglial Reactivity Protects from Acute and Progressive Neuronal Dysfunction, Motor Impairments and Sedation following Alcohol Abuse.

bioRxiv : the preprint server for biology·2025

Same author

Cisplatin-Induced APE2 Overexpression Disrupts MYH9 Function and Causes Hearing Loss.

Cancer research communications·2025

Same author

Amyloid-β induces lipid droplet-mediated microglial dysfunction via the enzyme DGAT2 in Alzheimer's disease.

Immunity·2025

Same author

Rapid postradiation malignant transformation of a pilocytic astrocytoma in an adult with neurofibromatosis type 1: illustrative case.

Journal of neurosurgery. Case lessons·2024

Same author

Long non-coding RNA lung cancer-associated transcript-1 promotes glioblastoma progression by enhancing Hypoxia-inducible factor 1 alpha activity.

Neuro-oncology·2024

Same author

Clinical presentation and extent of resection impacts progression-free survival in spinal ependymomas.

Journal of neuro-oncology·2024

Area of Science:

Neurology
Pathology
Muscle Diseases

Background:

Skeletal muscle disorders can exhibit regional variability.
Simultaneous biopsy of multiple muscles may improve diagnostic accuracy.
Limited literature supports the efficacy of multi-site muscle biopsy.

Purpose of the Study:

To evaluate the diagnostic utility of simultaneous multi-muscle biopsies.
To determine the concordance of diagnoses between simultaneously biopsied muscles.
To assess the impact of multi-site biopsy on diagnosing inflammatory myopathies.

Main Methods:

Retrospective review of 99 cases over 8 years.
Analysis of clinical symptoms and diagnoses from simultaneous muscle biopsies.
Comparison of diagnoses between two biopsied muscles per case.

More Related Videos

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Published on: February 7, 2015

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Published on: September 27, 2020

Related Experiment Videos

Last Updated: Jul 19, 2026

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Human Vastus Lateralis Skeletal Muscle Biopsy Using the Weil-Blakesley Conchotome

Published on: March 4, 2016

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Published on: February 7, 2015

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Published on: September 27, 2020

Main Results:

Diagnoses were consistent in both muscles in 55% of cases.
A diagnosis was obtained from only one biopsy in 17% of cases.
For inflammatory myopathies, 34% of diagnoses required only one biopsy, highlighting potential missed diagnoses with single-site biopsies.

Conclusions:

Simultaneous multi-muscle biopsy increases the likelihood of diagnosing skeletal muscle disorders.
Biopsying two sites is justified in suspected inflammatory myopathies to avoid missed diagnoses.
This approach enhances diagnostic yield for regional muscle pathologies.