Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A novel cystic fibrosis-mimetic Pseudomonas auxotrophic vaccine is protective in vivo and is associated with IL-17/IgA mucosal Immunity.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

Same author

Integrative metagenomics and metabolomics reveal age-associated gut microbiota and metabolite alterations in a hamster model of COVID-19.

Gut microbes·2025

Same author

Integrative metagenomics and metabolomics reveal age-associated gut microbiota and metabolite alterations in experimental COVID-19.

bioRxiv : the preprint server for biology·2024

Same author

Rubrolone production by Dactylosporangium vinaceum: biosynthesis, modulation and possible biological function.

Applied microbiology and biotechnology·2021

Same author

[Twenty years of on-site clinical genetics consultations for people with ASD].

Medecine sciences : M/S·2019

Same author

Use of whole-genome sequencing in the molecular investigation of care-associated HCoV-OC43 infections in a hematopoietic stem cell transplant unit.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2019

Search research articles

Related Experiment Video

Updated: Jul 19, 2026

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Methods and platforms for the quantification of splice variants' expression.

Laurent Bracco¹, Emeline Throo, Olivier Cochet

¹ExonHit Therapeutics, 65 Boulevard Masséna, F-75013 Paris.

Progress in Molecular and Subcellular Biology

|November 2, 2006

Summary

Understanding mRNA splice isoforms is crucial due to limited human genes. This work reviews transcriptome analysis techniques for quantifying splice isoform expression levels.

More Related Videos

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Related Experiment Videos

Last Updated: Jul 19, 2026

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Area of Science:

Molecular Biology
Genomics
Transcriptomics

Background:

The human genome has a limited number of protein-encoding genes, making mRNA transcript diversity essential for biological complexity.
Alternative RNA splicing is a primary mechanism generating this diversity, leading to multiple mRNA variants from a single gene.
Accurate quantification of splice isoform expression is critical for understanding gene regulation and cellular function.

Purpose of the Study:

To highlight the importance of mRNA transcript diversity in the context of a limited human gene set.
To discuss the critical need for tools and platforms that provide quantitative data on splice isoform expression levels.
To review the current state-of-the-art techniques and available products for global transcriptome analysis.

Main Methods:

The chapter focuses on the challenges and limitations inherent in global transcriptome analysis.
It reviews existing and emerging technologies for analyzing and quantifying splice isoforms.
Discussion includes the practical aspects and availability of these techniques for researchers.

Main Results:

Transcriptome analysis faces specific constraints that impact the accurate measurement of splice isoforms.
Several state-of-the-art techniques are available, offering varying degrees of resolution and quantitative accuracy.
The landscape of tools and platforms is evolving to meet the demand for precise splice isoform quantification.

Conclusions:

The diversity generated by alternative RNA splicing is fundamental to human biology.
Developing and utilizing advanced transcriptome analysis tools is paramount for accurate splice isoform quantification.
The scientific community has access to a growing array of techniques to study splice isoform expression levels.