Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse enzyme synthesis in microemulsion-based organo-gels.

G D Rees1, M da Graca Nascimento, T R Jenta

  • 1School of Chemical Sciences, University of East Anglia, Norwich, U.K.

Biochimica Et Biophysica Acta
|April 9, 1991
PubMed
Summary

Novel solid-phase catalysts using immobilized lipases in microemulsion-based gels (MBGs) enable efficient ester synthesis. These robust catalysts offer high yields, stereoselectivity, and stability for preparative applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cadmium uptake by onions, lettuce and spinach in New Zealand: Implications for management to meet regulatory limits.

The Science of the total environment·2019
Same author

Biowastes to augment the essential oil production of Leptospermum scoparium and Kunzea robusta in low-fertility soil.

Plant physiology and biochemistry : PPB·2019
Same author

The potential of L. scoparium, K. robusta and P. radiata to mitigate N-losses in silvopastural systems.

Environmental pollution (Barking, Essex : 1987)·2017
Same author

Response of Leptospermum scoparium, Kunzea robusta and Pinus radiata to contrasting biowastes.

The Science of the total environment·2017
Same author

Production of Biomass Crops Using Biowastes on Low-Fertility Soil: 1. Influence of Biowastes on Plant and Soil Quality.

Journal of environmental quality·2016
Same author

Production of Biomass Crops Using Biowastes on Low-Fertility Soil: 2. Effect of Biowastes on Nitrogen Transformation Processes.

Journal of environmental quality·2016

Area of Science:

  • Biocatalysis
  • Enzyme immobilization
  • Organic synthesis

Background:

  • Enzyme immobilization is crucial for developing reusable and stable catalysts.
  • Microemulsion-based gels (MBGs) offer a promising matrix for enzyme encapsulation.
  • Lipases are versatile enzymes for ester synthesis and chiral resolutions.

Purpose of the Study:

  • To immobilize lipases in MBGs for use as solid-phase catalysts.
  • To evaluate the catalytic activity, stability, and preparative potential of these immobilized lipases.
  • To investigate the stereoselectivity of lipase-catalyzed esterification reactions.

Main Methods:

  • Immobilization of three different lipases within microemulsion-based gels (MBGs).
  • Evaluation of catalytic activity in apolar organic solvents (e.g., n-heptane).

Related Experiment Videos

  • Preparative-scale synthesis of various esters and stereoselective esterification of octan-2-ol.
  • Assessment of catalyst reusability and stability over time and at low temperatures.

    • Main Results:

    • Lipases immobilized in MBGs retained high catalytic activity.
    • MBGs served as effective solid-phase catalysts for ester synthesis in apolar solvents, yielding products in high yields.
    • Chromobacterium viscosum (CV) lipase demonstrated significant stereoselectivity, achieving 92% enantiomeric excess in octan-2-ol esterification.
    • The CV lipase-containing MBG showed 75% of its initial activity after 30 days and was effective at -20 degrees C.
    • MBGs were suitable for column flow reactors.

    Conclusions:

    • Lipase-containing MBGs are novel, reusable solid-phase catalysts for efficient and stereoselective ester synthesis.
    • These immobilized enzyme systems offer advantages for preparative organic synthesis under mild conditions.
    • The developed MBG catalysts exhibit excellent stability and versatility for various applications, including flow chemistry.