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Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Docking interactions in protein kinase and phosphatase networks
Attila Reményi1, Matthew C Good, Wendell A Lim
1Department of Cellular and Molecular Pharmacology, Program in Biological Sciences, University of California San Francisco, 600 16th Street, San Francisco, CA 94143-2240, USA.
Current Opinion in Structural Biology
|November 3, 2006
Summary
Protein kinases and phosphatases use docking interactions, recognizing short peptide motifs outside the active site, to achieve high biological specificity. This mechanism is crucial for building complex signaling networks and enabling allosteric regulation.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Signaling
Background:
- Protein kinases and phosphatases are key regulators of cellular processes.
- While active site interactions are known, non-active site interactions are increasingly recognized.
- Modular protein-protein interaction domains are well-studied in signaling.
Purpose of the Study:
- To highlight the significance of docking interactions in protein kinase and phosphatase function.
- To explain how docking interactions contribute to biological specificity.
- To explore the role of docking in signaling network organization and regulation.
Main Methods:
- Literature review and synthesis of existing research on protein-protein interactions in kinases and phosphatases.
- Analysis of structural and functional data related to docking interactions.
- Comparative analysis of docking prevalence across different kinase and phosphatase families.
Main Results:
- Docking interactions, involving recognition of peptide motifs by catalytic domain grooves, are prevalent in serine/threonine kinases and phosphatases.
- These interactions occur outside the active site, conferring high target specificity.
- Docking serves as a versatile organizational tool for complex signaling networks.
Conclusions:
- Docking interactions are a critical mechanism for achieving biological specificity in kinase and phosphatase signaling.
- This mode of recognition facilitates the construction of intricate signaling pathways.
- Docking can also mediate allosteric regulation, adding another layer of control to signaling networks.
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