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Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
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Updated: Jul 19, 2026

High-throughput Screening for Protein-based Inheritance in S. cerevisiae
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Published on: August 8, 2017

Prion interference is due to a reduction in strain-specific PrPSc levels.

Jason C Bartz1, Michelle L Kramer, Meghan H Sheehan

  • 1Department of Medical Microbiology and Immunology, Creighton University, Omaha, Nebraska 68178, USA. jbartz@creighton.edu

Journal of Virology
|November 3, 2006
PubMed
Summary

When two prion strains infect the same neurons, one can block the other

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Last Updated: Jul 19, 2026

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Protein Misfolding Cyclic Amplification of Prions
10:12

Protein Misfolding Cyclic Amplification of Prions

Published on: November 7, 2012

Area of Science:

  • Neuroscience
  • Infectious Diseases
  • Molecular Biology

Background:

  • Prion diseases involve misfolded prion proteins (PrPSc).
  • Coinfection with different prion strains can lead to complex interactions.
  • Strain interference in prion disease pathogenesis is not fully understood.

Purpose of the Study:

  • To investigate prion strain interference at the replication level.
  • To determine if one prion strain can inhibit the replication of another.
  • To explore the role of neuronal targeting in prion strain interference.

Main Methods:

  • Hamsters were inoculated with two transmissible mink encephalopathy (TME) strains: DY TME (long incubation) and HY TME (short incubation).
  • Inoculations were performed via the sciatic nerve to target common neuronal populations in the lumbar spinal cord.
  • Prion protein (PrPSc) levels for both strains were quantified in the lumbar spinal cord.

Main Results:

  • Increased intervals between DY TME and HY TME inoculations extended HY TME incubation periods or blocked disease.
  • DY TME PrPSc was detected in the lumbar spinal cord, correlating with extended HY TME incubation.
  • Reduced HY TME PrPSc abundance was observed in coinfected hamsters, indicating inhibited replication.
  • Interference did not occur when strains targeted different neuronal populations.

Conclusions:

  • DY TME can interfere with HY TME replication when they infect the same neurons.
  • Prion strain interference is dependent on shared neuronal targets.
  • This suggests a mechanism of competitive inhibition between prion strains within host neurons.