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Updated: Jul 19, 2026
Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
Published on: June 2, 2023
Langerhans cells in cutaneous tumours: immunohistochemistry study using a computer image analysis system
Mario Ribeiro De Melo1, Jorge Luiz Araújo Filho, Vasco José Ramos Patu
1Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Av. Morais Rêgo s/n, Campus Universitário, 50670-910 Recife, PE, Brazil. mariormj@gmail.com
Abstract:
Immunohistochemistry, based on antibody anti-S100 protein, was used to evaluate the Langerhans cells (LC) in benign and malign skin neoplasias. These cells were quantitatively estimated using a computer image analysis (OPTIMAS software system, Version 6.1) in skin biopsies diagnosed as basal cell carcinoma (BCC), epidermoid carcinoma (EpC), trichoepithelioma (TE), keratoacanthoma (KA), seborreic keratosis (SK) and actinic keratosis (AK). The antibody anti-S100 protein recognized them. No significant variations were observed in the number of LC among malignant tumour (BCC = 23.25 +/- 5.81 and EpC = 20.88 +/- 4.24). Benign lesions (AK = 33.04 +/- 7.11; TE = 55.74 +/- 9.35; SK = 42.38 +/- 9.92, and KA = 47.62 +/- 10.4) presented a higher number of LC when they were compared among them and to malignant and normal tissues. No significant differences were observed in LC area and volume between benign and malign neoplasias. These results indicate possibly differences in the immunogenicity between benign and malign epidermic tumours. In conclusion, the experimental computer assessment method was reliable and consistent to morphometric analysis of tumoural tissues.
