[Influence of meliorated AS-PCR primer for ABO genotyping]
Xin-Jie Wang1, Li-Jiang Diao, Jian-Zhong Feng
1Weifang Public Security Bureau, Weifang 261061, China.
Fa Yi Xue Za Zhi
|November 4, 2006
Abstract
Objective:
To improve the correct rate of ABO genotyping by meliorating AS-PCR primer.
Methods:
The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed.
Results:
The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer.
Conclusion:
The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.


