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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

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Related Experiment Video

Updated: Jul 19, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Capillary array reversed-phase liquid chromatography-based multidimensional separation system coupled with

Xue Gu1, Chunhui Deng, Guoquan Yan

  • 1Department of Chemistry & Research Center of Proteome, Fudan University, Shanghai 200433, China.

Journal of Proteome Research
|November 4, 2006
PubMed
Summary

A novel high-throughput proteomic analysis system significantly increases throughput by using 18 parallel liquid chromatography (LC) columns. This advanced system enables comprehensive proteomic analyses with enhanced efficiency.

Related Experiment Videos

Last Updated: Jul 19, 2026

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery
13:00

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Comprehensive proteomic analyses are crucial for understanding biological systems.
  • Existing two-dimensional liquid chromatography (2D-LC) systems can be limited by throughput.
  • High-throughput methods are needed for efficient large-scale proteomic studies.

Purpose of the Study:

  • To develop a high-throughput on-line capillary array-based 2D-LC system coupled with MALDI-TOF-TOF-MS.
  • To enhance the efficiency and speed of proteomic analyses.
  • To enable comprehensive analysis of complex biological samples.

Main Methods:

  • A 2D-LC system was constructed using one strong-cation exchange (SCX) column and 18 parallel reversed-phase liquid chromatography (RPLC) columns.
  • Peptides were fractionated using salt pulses on the SCX column.
  • Peptide fractions were simultaneously separated using the 18 parallel RPLC columns.
  • Effluents were directly deposited onto MALDI target plates for mass spectrometry analysis.

Main Results:

  • The developed system demonstrated an 18-fold increase in throughput compared to serial 2D-LC systems.
  • Analysis of human liver tissue digest identified a total of 462 proteins.
  • The system proved highly efficient for comprehensive proteomic analysis.

Conclusions:

  • The capillary array-based 2D-LC-MALDI-TOF-TOF-MS system offers a significant advancement in high-throughput proteomics.
  • This technology holds promising potential for large-scale proteomic studies and applications.
  • The system enables efficient and comprehensive protein identification from complex biological samples.