Related Experiment Video
Updated: Jul 19, 2026

Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader
Published on: May 24, 2024
[C-reactive protein induced human endothelium cells apoptosis is associated with Bcl-2/Bax gene expression changes]
Xing-wei Zhang1, Jun-bo Ge, Hao Pan
1Department of Cardiology, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200032, China.
Objective:
In the present study, we examined the expression changes of Bcl-2/Bax in C-reactive protein (CRP) treated human endothelium cells in vitro.
Methods:
The human umbilical vein endothelial cells (HUVEC) were cultured by digest method for 2 - 3 posterities and incubated with human CRP (0, 1, 5, 25 mg/L for 24 hours) and analyzed by flow cytometer for apoptosis ratio. The effects of CRP in various concentrations on Bcl-2/Bax mRNA and protein expression were examined by RT-PCR and Western Blotting.
Results:
Apoptosis ratio increased, downregulated Bcl-2 (gene promoting cell survival) and upregulated Bax (gene promoting apoptosis) at mRNA and protein levels in proportion to increased CRP concentrations.
Conclusion:
These results demonstrate that Bcl-2/Bax could be regulated by CRP in human HUVECs and might play a causal role in CRP-induced apoptosis.
Related Concept Videos
The Intrinsic Apoptotic Pathway
Cellular Injury V: Apoptosis and Autophagy
The Extrinsic Apoptotic Pathway
Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
Regulation of Angiogenesis and Blood Supply
