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Updated: Jul 19, 2026
In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model
Published on: January 21, 2018
[Fluorescein angiography of retinal neovascularization model in mice]
Yi-chun Kong1, Mei Han, Kan-xing Zhao
1Tianjin Medical University, Tianjin Eye Hospital, Tianjin 300022, China.
Objective:
To investigate a high molecular weight fluorescein angiography in the application of retinal neovascularization model in mouse.
Methods:
retinal neovascularization model was induced by exposure mouse to an environment containing high concentration of oxygen. High molecular weight fluorescein isothiocyanate dextran were perfused through the left ventricle directly, then the mouse eyes were enucleated and fixed with 4% paraformaldehyde. The retina was separated from the eyecup and flat mounting was performed on a gelatin coated slide. The vasculature was examined under fluorescent microscope.
Result:
The whole retinal vasculature was clearly visualized under fluorescent microscope. By focusing on different layer of the tissue, superficial, deep vascular plexus and connecting vessels also could be distinguished. The neovascular response occurred at the junction between the vascular and the avascular retina.
Conclusion:
High molecular weight fluorescein angiography can be applied for retinal neovascularization evaluation.
