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Updated: Jul 19, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Flavobacterium indicum sp. nov., isolated from warm spring water in Assam, India
1Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Sector 39A, Chandigarh 160 036, India.
Abstract:
A polyphasic taxonomic approach was employed to characterize a strain designated GPTSA100-9T, which was isolated from water sampled from a warm spring. The micro-organism, comprising Gram-negative, strictly aerobic rods, could not grow on nutritionally rich media such as tryptic soy broth. Analysis of the 16S rRNA gene sequence (1396 nt) of strain GPTSA100-9T revealed that it is a member of the genus Flavobacterium, sharing 99.8% sequence similarity with the CFB group bacterium strain A0653 (AF236016), 93.4% with '[Flexibacter] aurantiacus subsp. excathedrus' and 93.2-92.0% with Flavobacterium saliperosum, Flavobacterium soli, Flavobacterium aquatile and Flavobacterium columnare. The G+C content of the genomic DNA was 31.0 mol%. The major fatty acids of the strain grown on modified R2A agar were iso-C15:0 (18.5%), iso-C15:1 G (18.0%), summed feature 3 (iso-C15:0 2-OH and/or C16:1omega7c, 16.6%) and iso-C17:0 3-OH (9.0%). On the basis of phenotypic and genotypic characteristics, strain GPTSA100-9T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium indicum sp. nov. is proposed. The type strain is GPTSA100-9T (=MTCC 6936T=DSM 17447T).
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