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Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
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Published on: August 7, 2014

Major basic protein homolog (MBP2): a specific human eosinophil marker.

Douglas A Plager1, David A Loegering, James L Checkel

  • 1Allergic Diseases Research Laboratory, Mayo Clinic and Foundation, Rochester, MN 55905, USA. plager.douglas@mayo.edu

Journal of Immunology (Baltimore, Md. : 1950)
|November 4, 2006
PubMed
Summary

Major basic protein 2 (MBP2) is a novel eosinophil protein. Unlike MBP1, MBP2 is found exclusively in eosinophils and may serve as a valuable biomarker for eosinophil-associated diseases.

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Published on: October 15, 2021

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Last Updated: Jul 19, 2026

Hydrogel Nanoparticle Harvesting of Plasma or Urine for Detecting Low Abundance Proteins
10:05

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Published on: August 7, 2014

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Human eosinophil granules contain major basic protein (MBP1), a highly cationic protein.
  • A less cationic homolog, major basic protein 2 (MBP2), has been recently identified.

Purpose of the Study:

  • To develop and utilize monoclonal antibodies (mAbs) for the localization and quantification of MBP2.
  • To compare the distribution and abundance of MBP1 and MBP2 in various cell types and biological fluids.

Main Methods:

  • Development of specific mAbs against MBP2.
  • Application of slot-blot, two-site immunoassay, Western blotting, and immunofluorescence microscopy.
  • Optimization of immunoassay sensitivity through reduction and alkylation.

Main Results:

  • MBP2 is exclusively detected in eosinophils, unlike MBP1 which is also found in basophils and mast cells.
  • MBP1 is more abundant than MBP2 in eosinophil lysates and granules.
  • Serum MBP2 levels effectively differentiated patients with elevated eosinophils from normal subjects and were detectable in other biological samples.

Conclusions:

  • MBP2 is a specific marker for eosinophils.
  • MBP2 shows potential as a biomarker for diagnosing and monitoring eosinophil-associated diseases.