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Molecular characterization of a severe isolate of papaya ringspot virus in Mexico and its relationship with other
Juan Carlos Noa-Carrazana1, Diego González-de-León, Laura Silva-Rosales
1Cinvestav Campus Guanajuato, Apdo Postal 629, Irapuato, Gto, 36500, Mexico.
Abstract:
The virus most often reported in papaya (Carica papaya L.) is papaya ringspot (PRSV). The aim of this work was the molecular genomic characterization of a Mexican severe isolate of PRSV-P "Mex-VrPO" (isolate from the State of Veracruz in Paso de Ovejas) as well as its comparison with other isolates from other world regions. The linear, assembled, single-strand positive sense RNA genome of PRSV-P Mex-VrPO was 10320 nt in length (excluding the poly(A) tail) and contained a single large predicted ORF with 3344 aa. The comparative analysis of our PRSV isolates and five others reported before, showed the most variable proteins were P1, P3, 6 K and CP with 13-33%, 5-7%, 6-9% and 5-9% divergence respectively. The most conserved ones were CI, NIb and HC-Pro (2-3%, 3-5% and 4-5%). The phylogenetic analysis showed a close relation between the Mexican (Mex-VrPO) and Hawaiian (PRSV-P HA) isolates. This work provided the first opportunity to establish the foundation for (1) understanding whole genome and polyprotein variability between Asian and American PRSV isolates, and (2) elucidating major trends in the relative evolution of viral cistrons as deduced from in silico recombination analyses.
Insights
This study characterized the Mexican papaya ringspot virus (PRSV) genome, revealing its genetic variability compared to global isolates. Findings provide insights into PRSV evolution and inform strategies against this damaging papaya virus.
Area of Science:
- Plant Virology
- Molecular Biology
- Genomics
Background:
- Papaya ringspot virus (PRSV) is a major pathogen affecting papaya (Carica papaya L.) cultivation worldwide.
- Understanding PRSV genetic diversity is crucial for developing effective disease management strategies.
Purpose of the Study:
- To perform molecular genomic characterization of a severe Mexican PRSV-P isolate (Mex-VrPO).
- To compare the Mexican isolate with other global PRSV strains.
- To analyze viral protein variability and evolutionary trends.
Main Methods:
- Genome sequencing and assembly of the PRSV-P Mex-VrPO isolate.
- Comparative genomic analysis with existing PRSV sequences.
- Phylogenetic analysis to determine evolutionary relationships.
- In silico recombination analysis.
Main Results:
- The PRSV-P Mex-VrPO genome is a 10320 nt positive-sense single-stranded RNA with a single large open reading frame (ORF).
- Proteins P1, P3, 6K, and coat protein (CP) exhibited the highest variability (13-33%), while CI, NIb, and HC-Pro were most conserved (2-5%).
- Phylogenetic analysis indicated a close relationship between the Mexican Mex-VrPO and Hawaiian PRSV-P HA isolates.
Conclusions:
- This research provides the first comprehensive genomic characterization of a Mexican PRSV-P isolate.
- The study establishes a foundation for understanding genomic variability between Asian and American PRSV isolates.
- It elucidates evolutionary trends in viral cistrons, aiding in the development of resistant papaya varieties.
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