Related Experiment Video
Updated: Jul 19, 2026
11:16
A Microfluidic Chip for ICPMS Sample Introduction
Published on: March 5, 2015
Multitrack electrospray chips.
Loïc Dayon1, Mélanie Abonnenc, Michel Prudent
1Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 6, CH-1015 Lausanne, Switzerland.
Journal of Mass Spectrometry : JMS
|November 4, 2006
Summary
Multitrack electrospray chips (MTEC) enable fast screening of peptides and proteins using mass spectrometry. This novel tool simplifies analysis by eliminating the need for chip alignment during sequential electrospraying.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Materials Science
Background:
- Multitrack electrospray chips (MTEC) offer a novel approach for mass spectrometry (MS) analysis.
- Fabrication involves UV-photoablation of polyethylene terephthalate (PET) substrates.
Purpose of the Study:
- To evaluate the efficacy of MTEC for rapid screening of peptides and proteins.
- To introduce new analytical strategies utilizing MTEC for cysteine counting and peptide quantification.
Main Methods:
- Fabrication of MTEC with up to six microchannels using UV-photoablation.
- Sequential electrospraying from off-axis microchannels without MS orifice alignment.
- Application of high voltage for successive electrospray ionization (ESI).
Main Results:
- Demonstrated successful sequential MS analyses without moving the MTEC chip.
- Developed a method for counting cysteines in peptides via chemical modification.
- Established a strategy for relative peptide quantification across two samples.
Conclusions:
- MTEC provides a fixed, efficient platform for high-throughput peptide and protein analysis.
- The developed strategies enable advanced applications in chemical modification analysis and quantitative proteomics.
Related Concept Videos
01:12Electrospray Ionization (ESI) Mass Spectrometry
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
01:26Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.