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Updated: Jul 19, 2026

Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
Published on: February 27, 2021
Arabinogalactan protein from Arachis hypogaea: role as carrier in drug-formulations
Seema Parveen1, Ashish D Gupta, Ramasare Prasad
1Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Abstract:
Arabinogalactan protein (AGP) a highly water-soluble glyco-conjugate from groundnut (Arachis hypogaea L.) seedling was isolated and purified by precipitation with beta-glucosyl Yariv reagent. Quantification of AGP was done by gel diffusion assay. Purified AGP was conjugated to amphotericin-B (AmB) by Schiff base reaction at pH 11.0, with aim to prepare a water-injectable lesser toxic AGP-AmB conjugate without affecting AmB antifungal potential. The AGP-AmB conjugate antifungal activity was assayed by serial broth dilution and disc method against several Candida albicans clinical isolates. Both AmB and AGP-AmB showed similar MICs and MFCs activities, indicating that AGP do not reduced the antifungal activity of AmB. However, the in vitro and in vivo toxicity assays revealed that AGP-AmB conjugate was lesser toxic than AmB, as high MTD (45 mg/kg body weight) was observed. It is suggested that AGP could be a potent carrier in AmB formulation, which may result in effective treatment of fungal infections.
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