Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Epac proteins: multi-purpose cAMP targets.

Johannes L Bos1

  • 1Department of Physiological Chemistry and Centre for Biomedical Genetics, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands. j.l.bos@med.uu.nl

Trends in Biochemical Sciences
|November 7, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Drug-repurposing screen on patient-derived organoids identifies therapy-induced vulnerability in KRAS-mutant colon cancer.

Cell reports·2023
Same author

Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling.

Nature cell biology·2021
Same author

An organoid platform for ovarian cancer captures intra- and interpatient heterogeneity.

Nature medicine·2019
Same author

CRISPR-induced RASGAP deficiencies in colorectal cancer organoids reveal that only loss of NF1 promotes resistance to EGFR inhibition.

Oncotarget·2019
Same author

A Tuba/Cdc42/Par6A complex is required to ensure singularity in apical domain formation during enterocyte polarization.

PloS one·2018
Same author

The Phosphatase PTPL1 Is Required for PTEN-Mediated Regulation of Apical Membrane Size.

Molecular and cellular biology·2018
Same journal

Peptideins: Navigating the gray zone of the proteome.

Trends in biochemical sciences·2026
Same journal

A metabolon channels nicotine biosynthesis.

Trends in biochemical sciences·2026
Same journal

Better call chaperone.

Trends in biochemical sciences·2026
Same journal

Biochemistry at scale: Seeing both the forest and the trees.

Trends in biochemical sciences·2026
Same journal

Voices across Asia and Oceania: Biochemistry across borders.

Trends in biochemical sciences·2026
Same journal

Metabolic control of RNA splicing by polyamines.

Trends in biochemical sciences·2026
See all related articles

Epac1 and Epac2 are key cAMP signaling proteins. Their structure reveals activation mechanisms, highlighting roles in cell adhesion, insulin secretion, and neurotransmitter release, alongside protein kinase A (PKA).

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Biochemistry

Background:

  • Epac1 and Epac2 are cyclic adenosine monophosphate (cAMP)-dependent guanine-nucleotide exchange factors.
  • They regulate small GTPases Rap1 and Rap2, acting as crucial mediators in cAMP signaling pathways.
  • These proteins are involved in diverse cellular functions, including cell adhesion and secretion processes.

Purpose of the Study:

  • To elucidate the activation mechanism of multi-domain Epac proteins.
  • To understand the role of Epac proteins in various cellular processes.
  • To explore the interplay between Epac-mediated and protein kinase A (PKA)-mediated signaling.

Main Methods:

  • Crystal structure determination of Epac2.
  • Analysis of Epac protein function in cellular processes.

Related Experiment Videos

  • Investigating the cross-talk between Epac and PKA signaling pathways.

    • Main Results:

    • The crystal structure of Epac2 provided insights into its activation mechanism.
    • Epac proteins are implicated in integrin-mediated cell adhesion, cell-cell junction formation, insulin secretion, and neurotransmitter release.
    • Evidence suggests significant interconnectivity between Epac and PKA signaling pathways.

    Conclusions:

    • Epac proteins are vital regulators of cellular processes through cAMP signaling.
    • Understanding Epac activation mechanisms is key to deciphering their cellular roles.
    • The intricate relationship between Epac and PKA signaling pathways warrants further investigation.