Related Experiment Video
Updated: Jul 19, 2026
10:07
Isolation of Type I and Type II Pericytes from Mouse Skeletal Muscles
Published on: May 26, 2017
Characterization of pericentrin isoforms in vivo
Ko Miyoshi1, Masato Asanuma, Ikuko Miyazaki
1Department of Brain Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan. miyoshi@cc.okayama-u.ac.jp
Biochemical and Biophysical Research Communications
|November 7, 2006
Summary
Researchers identified novel pericentrin isoforms in mice, including pericentrin S. This study clarifies the in vivo products of pericentrin, a key protein in ciliogenesis.
Area of Science:
- Cell Biology
- Molecular Biology
Background:
- Pericentrin is a key centrosomal protein involved in ciliogenesis.
- Previous studies identified pericentrin A in mice and pericentrin B in humans, but in vivo products remained unclear.
Purpose of the Study:
- To characterize pericentrin isoforms and their expression in mice.
- To resolve ambiguities regarding in vivo pericentrin products.
Main Methods:
- Northern blotting to detect pericentrin transcripts.
- cDNA identification and sequencing.
- Immunoprecipitation analysis to identify pericentrin proteins.
Main Results:
- Two pericentrin transcripts (9.5 kb and 6.9 kb) were detected in mouse tissues.
- Two pericentrin cDNAs were identified, encoding pericentrin B and a novel isoform, pericentrin S.
- Three pericentrin proteins (360, 255, and 250 kDa) were identified, likely corresponding to pericentrin B, pericentrin S, and an N-terminal product.
Conclusions:
- The study identified and characterized novel pericentrin isoforms in mice, including pericentrin S.
- This work clarifies the in vivo molecular landscape of pericentrin, a crucial protein for ciliogenesis.
